| Kitsize | 12x8 |
| Method | ELISA |
| Incubationtime | 1x60min,1x30min,1x20min,1x15-20min |
| Standardrange | 10-1000ng/mL |
| Specimen/Volumes | Urine |
| Substrate/isotope | TMB450nm |
| RegulatoryStatus: | EU:CE |
TheonlycommerciallyavailableELISAassay
IBLInternationalprovidesareliableandfastimmunoassayforthedeterminationofthemainmetaboliteofhistamine,1-methylhistamine(N-methylhistamine),invariousBIOLOGicalfluids.
Extensiveresearchhasshownthathistamine[2-(4-imidazolyl)-ethylamine]istheprinciplemediatorofmultiplebiologicalprocessesincludingneuromodulation,inflammation,gastricacidsecretionandtheregulationofimmunefunction.Itismainlystoredinthemetachromaticgranulaofmastcellsandthebasophilicleukocytes,andreleaseduponstimulation.
HistamineispredominantlymetabolizedviaN-methytransfereseto1-methylhistamine,whichisfurtherprocessedtoN-methylimidazoleaceticacid,whiletheremainderismetabolizedbydiamineoxidasetoimidazoleaceticacid(Fig.1).Theurinary1-methylhistaminelevelcorrelateswellwiththehistaminelevelsinplasmaandurine(1,2),andismorestablethanhistamineinplasma.
Thedeterminationofurinary1-methylhistaminefindsitapplicationprimarilyinthediagnosisandmonitoringofmast-celldisorders,includingmastocytosis,anaphylaxis,andotherseveresystemicallergicreactions.
Uniquefeatures
- TheonlycommerciallyavailableELISA
- Sensitivity:urine:2.5ng/mL,plasmaandcellculture:0.13ng/mL
- CalibratedtoLC-MS/MS(Fig.2)
- Totalassaytimeisonly2hours
- Alsoapplicableforplasmaandcellculturesamples
Histaminemetabolism.Synthesisofhistamineviadecarboxylationofhistidine(1).Metabolismofhistamineeitherbydiamineoxidase(DAO)(2)orbyhistamine-N-methyltransferase(HNMT)(3).Negativefeedbackinhibitionofbothenzymesbytheirrespectiveproducts(4).FurtherprocessingofN-MethylhistaminebymonoamineoxidaseB(MAOB)(5)orbyDAO(6).3)
MethodcomparisonbetweentheIBLInternational1-methylhistamineELISAandLC-MS/MSshowsanexcellentcorrelation:IBLInternationalinternaldata.)
Literature
1.HermannK.etal.Measurementandcharacterizationofhistamineandmethylhistamineinhumanurineunderhistamine-richandhistamine-poordiets.IntArchAllergyImmunol.1993;101(1):13-9.
2.BelicA.etal.Interdependenceofhistamineandmethylhistaminekinetics:modellingandsimulationapproach.ComputBiolMed.1999Nov;29(6):361-75.
3.MaintzL.etal.Histamineandhistamineintolerance.AmJClinNutr2007;85:1185–96.
DistributedbyIBLInternational.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.
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