Kitsize | 12x8Tests |
Method | ELISA |
Incubationtime | 1x1h,1x30min |
Standardrange | 25-5000pg/mL |
Specimen/Volumes | 50µLSaliva |
Substrate/isotope | TMB,450nm |
RegulatoryStatus: | EU:CE |
ThenewIBLInternationalProgesteroneSalivaELISAiscalibratedtotheLC-MS/MSreferencemethodandtotheluminescenceImmunoassay(IBLProgesteroneSalivaLUM,RE62021).
Thisnewassayrequiresaslowas50µLSalivasamplevolumeandhasabroadstandardrangeupto5,000pg/mL.
OurSalivaELISAproductlinehasbeenrevisedsothatthemainsteroids(cortisol,testosterone,progesterone,DHEA)maybeprocessedinasimilarway:
Thankstothesimilarprocessingandassembling,theassayscaneasilybeper-formedincombination.
ThetwomainindicationsforprogesteronemeasurementsaretheCorpusLuteuminsufficiencyaswellastheanovulatoricalmenstrualcyclesinfemales.Inanti-agingmedicineitmightbenecessarytoevaluatetheProgesteronelevelbeforeandduringthereplacementtherapy.
Duetothepulsatiledynamicsofsteroidsecretionrepeatedsalivasamplingisrec-ommended.IBLrecommendscollecting3to5salivasampleswithin2hours.Inthelaboratory,equalvolumesoftheindividualsalivasamplescanbemixed.ThismixedsampleresultsinameanfreeProgesteronevalue,whichrepresentstheactivehormoneconcentrationinareproducibleway.
InformationfromtheInstructionsforUse
Measurementsobtainedbythisassaymaybeusedinthediagnosisandtreatmentofdisordersoftheovariesorplacentaandcanbeusedasanaidforpredictionofovulation.TheProgesteronelevelinsalivarepresentstheconcentrationoftheactivefreeProgesterone.
Progesterone(4-pregnene-3,20-dione)isaC21steroidhormonecontainingaketo-group(atC-3)andadoublebondbetweenC-4andC-5.Likeothersteroids,itissynthesizedfromcholesterolviaaseriesofenzyme-mediatedsteps.ThesteroidhormoneProgesteroneisafemalesexhormonewhich,inconjunctionwithestro-gens,regulatestheaccessoryorgansduringthemenstrualcycleanditisparticu-larlyimportantinpreparingtheendometriumfortheimplantationoftheblastocyteandinmaintainingpregnancy.Innon-pregnantwomenprogesteroneismainlysecretedbythecorpusluteumwhereasinpregnancytheplacentabecomesthemajorsource.Minorsourcesforprogesteronearetheadrenalcortexforbothsexesandthetestesformales.
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