Kitsize | 12x8 |
Method | ELISA |
Incubationtime | 1x1h,3x30min,1x15min |
Standardrange | cut-offindex |
Specimen/Volumes | 10µlserum |
Substrate/isotope | TMB450nm |
RegulatoryStatus: | EU:CE |
IBLInternationaloffersahighlysensitiveandspecificELISAtestintendedforthediagnosticwork-upofchikungunyafever,aninfectiousdiseasewhoseincidencehasbeenincreasingsteADIlyinrecentyears.Itscharacteristicsare:
Chikungunyafeverisaviralfebrileillnesstransmittedbyseveralmosquitospecies.Itmayleadtoseverearthralgiathatmaypersistforweekstomonths.Nevertheless,chikungunyafeverisessentiallyself-limitingandnon-fatal.ChikungunyahashistoricallybeenlimitedtocountriesinAfrica,theIndiansubcontinentandSoutheastAsia.However,manytouristareasarenowalsoaffectedbyextensiveoutbreaksandincreasingincidence,whichishowthevirusmadeitswaytotheWesternHemisphere,resultinginimportedcasesinanumberofEuropeancountries.In2006,severalimportedchikungunyacaseswerediagnosedinNorthernItalyduetothepresenceofcompetentmosquitovectorsforthechikungunyavirus.
Laboratorydiagnosisisgenerallyaccomplishedbytestingseraumorplasmatodetectvirus-specificimmunoglobulin(IgM,IgG)bymeansofanimmunoassay.Sincethesymptomsandgeographicspreadofchikungunyaanddenguefeveroverlap,adifferentialdiagnosticwork-upisofutmostimportance,puttingspecialperformancedemandsupondiagnostictests.Moreover,duetotheabsenceofwidespreadSEROpositivityintheEuropeanpopulation,anypositiveantibodytestissUSPiciousforinfection.InadditiontotheChikungunyaIgMµ-captureELISA,IBLInternationaloffersanIgGcaptureELISA.Bothassaysboastoutstandingperformancedata.
1.ExternalmethodcomparisonoftheChikungunyaIgMµ-captureELISAofferedbyIBLInternationalwithanin-houseimmunofluorescencetest(TheBernhardNochtInstituteforTropicalMedicine,Hamburg)
Thecomparisonof31positiveandnegativeserarevealedaconcordanceof96.8%.
Immunofluorescence | |||
positive | negative | ||
IBLInternational | positive | 21 | 0 |
negative | 1 | 9 |
2.Analysisofselectedpositiveandnegativesera(DGHS,NewDelhi)usingtheIBLInternationalChikungunyaIgMµ-captureELISAassay
Theanalysisof88serarevealedanagreementof100%.
DGHS | |||
positive | negative | ||
IBLInternational | positive | 52 | 0 |
negative | 0 | 36 |
ExcerptfromtheInstructionsforUse
ChikungunyavirusisanarthropodbornevirusofthegenusAlphavirus(familyTogaviridae).TheAlphavirusgenuscontainsatleast24distinctspecies.Thesearelipid-envelopedvirionswithadiameterof50to60nm.Alphavirusinfectionsareinitiatedbythebiteofaninfectedmosquito,whichresultsinthedepositionofvirusinsubcutaneousandpossiblycutaneoustissues.Afteranincubationperiodof1to12daystheChikungunyafeverdevelops.Chikungunyafever(Chikungunyameans“thatwhichbendsup”,inreferencetothecripplingmanifestationsofthedisease)isanacuteviralinfectioncharacterizedbyarapidtransitionfromastateofgoodhealthtoillnessthatincludesseverearthralgiaandfever.Temperaturerisesabruptlytoashighas40°Candisoftenaccompaniedbyshakingchills.Afterafewdays,fevermayabateandrecrudesce,givingrisetoa“saddleback”fevercurve.Arthralgiaispolyarticular,favoringthesmalljointsandsitesofpreviousinjuries,andismostintenseonarising.Patientstypicallyavoidmovementasmuchaspossible.Jointsmayswellwithoutsignificantfluidaccumulations.Thesesymptomsmaylastfrom1weektoseveralmonthsandareaccompaniedbymyalgia.Therashcharacteristicallyappearsonthefirstdayofillness,butonsetmaybedelayed.Itusuallyarisesasaflushoverthefaceandneck,whichevolvestoamaculopapularormacularformthatmaybepruritic.Thelatterlesionsappearonthetrunk,limbs,face,plamsandsoles,inthatorderoffrequency.Petechialskinlesionshavealsobeennoted.Headache,photophobia,retro-orbitralpain,sorethroatwithobjectivesignsofpharyngitis,nauseaandvomitingalsooccurinthissetting.Occasionally,howeverpersistentarthralgiaandpolyarthritis(lastingmonthsorevenyears)dooccur,sometimesinvolvingjointdestruction.Evenrarer,sequelaeincludeencephalitisandmeningoencephalitiswithhighlethalityrates.ThevirushasmajorimportanceinAfricaandAsia.From20%tomorethan90%ofthepopulationoftropicalandsubtropicalshowserologicevidenceofinfection.BecauseAedesmosquitoesareincreasinglyprevalentinNorthAfricaandSouthAmerica,wherethepopulationwouldbeuniformlysusceptibletoinfection,thepossibilityforepidemicsisevident.ChikungunyavirusinfectionsareimportedtocentralEuropemainlybytravellerstotropicalandsubtropicalcountries.Thepresenceofvirusresp.infectionmaybeidentifiedbySerology:DetectionofantibodiesbyIF,ELISA
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