BoxAisoneofthehighlyconservedDNAbindingdomainsofHMGB1protein(HMGboxes).
BoxAisanantago
NISTforHMGB1bindingtoitsreceptorRAGE,andinhibitsHMGB1cytokineeffectsinvivo.TheBoxAweprovidehasnodifferencefromthesequenceofthehumanormouseHMGB1,andasaself-antigenelicitsverylowimmunologicalresponses.
BoxAprotectsagainstsepsisinamouseperitonitismodel(Yangetal.Reversingestablishedsepsiswithantagonistsofendogenoushigh-mobilitygroupbox1.ProcNatlAcadSciUSA2004,101:296-301),andfromhepatitisinamousemodelofHBVinfection(Sitiaetal.TreatmentwithHMGB1inhibitorsdiminishesCTL-inducedliverdiseaseinHBVtransgenicmice.JLeukocBiol2007,81:100-7).
DendriticcellspromotetheirownmaturationandTcellproliferation,survivalandTh1differentiationbysecretingHMGB1,andBoxAinhibitstheseactivities(Dumitriuetal.ReleaseofHMGB1bydendriticcellscontrolsTcellactivationviathereceptorforadvancedglycationendproducts(RAGE).JImmunol2005,174:7506-15).
BoxAalsoinhibitsthechemotacticactivityofHMGB1onendothelialcellsandfibroblasts,andthemitogenicactivityofHMGB1forstemcells(MEBianchi,unpublished).
ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.